Retrospective Study of Hemoparasites in Cattle in Southern Italy by Reverse Line Blot Hybridization
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چکیده
منابع مشابه
Retrospective Study of Hemoparasites in Cattle in Southern Italy by Reverse Line Blot Hybridization
Tick-borne diseases are widespread in tropical and temperate regions and are responsible for important economic losses in those areas. In order to assess the presence and prevalence of various pathogens in southern Italy, we retrospectively analyzed cattle blood samples collected for a previous study in 2000 using reverse line blot (RLB) hybridization. The study had been carried out in three re...
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Multiplex PCR and Reverse Line Blot Hybridization Assay (mPCR/RLB)
Multiplex PCR/Reverse Line Blot Hybridization assay allows the detection of up to 43 molecular targets in 43 samples using one multiplex PCR reaction followed by probe hybridization on a nylon membrane, which is re-usable. Probes are 5' amine modified to allow fixation to the membrane. Primers are 5' biotin modified which allows detection of hybridized PCR products using streptavidin-peroxidase...
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ژورنال
عنوان ژورنال: Journal of Veterinary Medical Science
سال: 2014
ISSN: 0916-7250,1347-7439
DOI: 10.1292/jvms.13-0365